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Editor: Stephanie J. Kok Updated: 10/24/2022 7:12:42 PM


Culdocentesis was, at one point, a mainstay for evaluation and diagnosis of hemoperitoneum that has largely been replaced by the increasing availability of high-resolution transvaginal sonography.[1] Culdocentesis is still widely used in developing countries that may not have access to sonography, such as Papua New Guinea, where it is the most common aid for the diagnosis of ruptured ectopic pregnancy.[2] 

It is especially important for diagnosis in countries with high rates of anemia and pelvic inflammatory disease as these can mimic ruptured ectopic pregnancy.[3] Culdocentesis has been important in the diagnosis of ruptured ectopic pregnancy, especially in the days before sonography. Ruptured ectopic pregnancy is considered to be a surgical emergency, and prompt diagnosis is critical.[4] 

Culdocentesis can also aid in the diagnosis of other conditions. For example, acute salpingitis or pelvic inflammatory disease will have purulent peritoneal fluid. Endometrioma will have "chocolate" fluid, and ascitic fluid return can be seen in other conditions.[3] Hemoperitoneum can be present in multiple pathologies and can cause false-positive results such as hemorrhagic ovarian cyst, torsion of an ovarian cyst, and ruptured ovarian follicle.

A negative culdocentesis (clear or serosanguinous fluid return) does not exclude ectopic pregnancy, and repeat culdocentesis at a later time may be needed. A negative culdocentesis supports that there is likely no hemoperitoneum and the fallopian tube is intact, but an ectopic pregnancy may still exist that has not yet ruptured. Almost 25% to 30% of ectopic pregnancies result in hemoperitoneum and is a significant cause of morbidity and mortality of reproductive-aged women.[2]

Anatomy and Physiology

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Anatomy and Physiology

On the sagittal cross-section of the female pelvis, the urinary bladder sits most anterior just behind the pubic bone. Moving posteriorly lies the uterus, which lays on top of the urinary bladder. The long axis of the uterus is perpendicular to the long axis of the vagina and where these two lines meet is the location of the cervix. There is a potential space just posterior to the uterus known as the pouch of Douglas (rectouterine pouch or cul-de-sac) that is separate from the uterus by the rectouterine septum. The pouch of Douglas is where blood can potentially pool if there is bleeding within the peritoneal cavity. Behind the pouch of Douglas is the rectum.


Indications include clinical suspicion for ruptured ectopic pregnancy and hemoperitoneum. Signs and symptoms include positive pregnancy test or elevated beta-human chorionic gonadotropin, abdominal pain, vaginal bleeding, peritoneal signs such as rigidity and rebound, rectal pressure, and hemodynamic instability, namely tachycardia, and hypotension.


Contraindications include a known mass or retroverted uterus that occupies the pouch of Douglas.[1][5]


Equipment includes speculum, lubricated jelly, a Foerster sponge forceps (ring forceps), sterile gauze/sponges, iodine solution, a tenaculum (or Vulsellum forceps), 18 gauge spinal needle (5 cm long), 10 mL syringe (three-finger control syringe), lidocaine, and gloves.[5]


Proceduralists (physician, physician assistant, or nurse practitioner) and a nurse or nursing aide are needed to carry out the procedure.


To prepare for a culdocentesis, have the supplies ready on a table near the proceduralist. Use good sterile technique when preparing supplies. Have a nurse or nursing aide present in the room to help hand over supplies. The patient should lay in the lithotomy position, preferably on a cart with stirrups for patient comfort.

Technique or Treatment

The procedure should start with a bimanual examination to assess the adnexa, cervix for cervical motion tenderness, uterus including uterine position, and for cul-de-sac mass or tenderness. Then a speculum exam should be performed by lubricating a speculum and inserting it into the vagina. Foerster sponge forceps should be used to introduce iodine soaked gauze to cleanse the cervix and posterior fornix.

Apply a tenaculum or Vulsellum forceps to the lower lip of the cervix, be sure to warn patients about potential cramps during this part of the procedure. Move the tenaculum to expose the posterior fornix of the vagina. Usually, this involves moving the cervix forward and anterior. Anesthetize the mucosa about 1 cm below the posterior rim of the cervix with lidocaine. Use an 18 gauge 10 cm long needle attached to a 10 mL syringe with 2 to 3 mL of air or sterile saline and insert the needle tip 1 cm below where the cervix ends in the posterior fornix. Advance the needle 3 to 4 cm and inject the air or saline. If there is resistance when injecting the air or saline, reposition the needle until there is no resistance felt. The needle should enter the Pouch of Douglas (rectouterine pouch or cul-de-sac). Aiming the needle towards the sacrum and away from the uterus may help with positioning. Lastly, attempt to aspirate. The procedure should be stopped if three attempts produce no fluid return.[3][6][5]


Complications include accidental puncture of visceral organs, the rectum, or uterus. Puncture of blood vessels, cysts, or tumors. Aspiration of products of an ectopic pregnancy, peritoneal introduction of infected or malignant cancer carrying cells.[1][5]

Clinical Significance

Once the aspirated peritoneal fluid is obtained, it can be analyzed microscopically and cultured.[3] Blood that is retrieved from the pouch of Douglas from the hemoperitoneum does not clot due to the process of defibrination. If the bleeding is brisk, however, the blood does clot. A positive culdocentesis is determined by the aspirated blood having a hematocrit of 0.15 or greater (15 % or greater). A non-diagnostic culdocentesis occurs when clotted blood is obtained, or no fluid is aspirated at all. If the needle inadvertently punctures an artery or vein, the blood will clot.[3] 

False-negative culdocentesis can occur in non-ruptured ectopic pregnancies or if the Pouch of Douglas is destroyed from endometriosis or scarring due to pelvic inflammatory disease.[7] Most notably, a negative culdocentesis should not exclude the diagnosis of ruptured ectopic. The further away from ectopic implants from the body of the uterus, the less extensive the bleeding that will occur. Likewise, the closer the ectopic is to the body of the uterus, the more significant the amount of bleeding.[8] 

In a comparative study performed in 1998, researchers set out to identify if culdocentesis was comparable to sonography in the diagnosis of hemoperitoneum in 46 patients, 40 of which had an ectopic pregnancy. The results of the study found that ultrasound showed 100% specificity and 100% sensitivity in identifying hemoperitoneum. Culdocentesis had 80% specificity and 66% sensitivity. The negative predictive value of a non-diagnostic culdocentesis was 25%. The negative predictive value of sonography was 100% when there was no fluid found on ultrasound.[1] Thus, culdocentesis has fallen out of favor in comparison to ultrasound for aiding in the diagnosis of hemoperitoneum because sonography has higher sensitivity and specificity than culdocentesis.

Enhancing Healthcare Team Outcomes

Rapid diagnosis of ruptured ectopic pregnancy is paramount to good patient outcomes. In high socioeconomic countries, ectopic pregnancy accounts for 1% to 2% of pregnancies, and it remains a leading cause of early maternal morbidity.[2] It takes multiple people from different professions to come together to diagnose, manage, and treat ruptured ectopic pregnancy. It often starts with nurses and emergency medical service providers that monitor the patient's vital signs and administer pain medications and fluids. Often these patients are hemodynamically unstable, and abnormal vital signs such as hypotension and tachycardia are the first indicators that there is a rupture. Next, the emergency physician evaluates the patient and is often making the diagnosis, but patient care does not stop there.

Laboratory technicians need to process labwork in a timely matter, including the beta-human chorionic gonadotropin level, hemoglobin, and view fluid under microscopy. Blood blank personnel may need to be involved if the patient has hemorrhagic shock and requires blood products. Pharmacists are involved in dosing pain medications and/or tranexamic acid. Radiologists interpret ultrasound images (if ultrasound is available) to aid in diagnosis.

Consulting physicians such as obstetric/gynecologic physicians or if obstetrics/gynecology is unavailable, general surgeons, need to be on stand by for emergent surgery if a patient presents with a ruptured ectopic. Often these patients need to be monitored for several days, and the physicians, advanced practice providers, nurses, and nurses' aids on the inpatient floors monitor and observe these patients. It truly takes a team to orchestrate the care for these patients, and each individual has an important role to play to ensure the best possible outcomes for these patients. [Level 1]



Chen PC, Sickler GK, Dubinsky TJ, Maklad N, Jacobi RL, Weaver JE. Sonographic detection of echogenic fluid and correlation with culdocentesis in the evaluation of ectopic pregnancy. AJR. American journal of roentgenology. 1998 May:170(5):1299-302     [PubMed PMID: 9574606]

Level 2 (mid-level) evidence


Hamura NN,Bolnga JW,Wangnapi R,Horne AW,Rogerson SJ,Unger HW, The impact of tubal ectopic pregnancy in Papua New Guinea--a retrospective case review. BMC pregnancy and childbirth. 2013 Apr 4;     [PubMed PMID: 23557190]

Level 2 (mid-level) evidence


Lucas C,Hassim AM, Place of culdocentesis in the diagnosis of ectopic pregnancy. British medical journal. 1970 Jan 24;     [PubMed PMID: 5412946]


. Culdocentesis and ectopic pregnancy. British medical journal. 1970 Jan 24:1(5690):187     [PubMed PMID: 5412942]


Murphy JT, General and Family Practice-Epitomes of Progress: Culdocentesis to Diagnose Ectopic Pregnancy and Pelvic Inflammatory Disease (PID). The Western journal of medicine. 1982 Jul;     [PubMed PMID: 18749144]


Tariq TA, Korejo R. Culdocentesis in diagnosis of disturbed ectopic pregnancy still a useful procedure in developing countries. JPMA. The Journal of the Pakistan Medical Association. 1992 Jan:42(1):5-6     [PubMed PMID: 1545483]


Wolfman W, Holtz G. Update on ectopic pregnancy. Canadian Medical Association journal. 1983 Dec 15:129(12):1265-9     [PubMed PMID: 6228291]


Weekes LR, Ectopic pregnancy: current clinical trends, a fifteen year study. Journal of the National Medical Association. 1981 Sep;     [PubMed PMID: 6456360]

Level 3 (low-level) evidence