Heterotopic ossification (HO) is a frequent complication in the rehabilitation setting which consists of the formation of mature, lamellar bone in the extraskeletal soft tissue where bone does not usually exist. Patient populations at risk of developing HO are those with burns, stroke, spinal cord injury (SCI), traumatic amputation, joint replacement, and traumatic brain injury (TBI). There are other genetic causes of HO such as fibrodysplasia ossificans progressiva, Albright’s hereditary osteodystrophy and progressive osseous heteroplasia that are not related to rehabilitation complications and will not be discussed here.
There are 2 main categories of HO: traumatic (fracture, arthroplasty, muscular trauma, joint dislocation, burns) versus neurogenic (stroke, SCI, TBI, brain tumors) . In total joint arthroplasty, HO most commonly occurs for the replacement of hips, knees, elbow, and shoulder. Chronic muscular trauma leads to what is traditionally known specifically as traumatic myositis ossificans. The most common sites for traumatic myositis ossificans are the quadriceps femoris muscle and the brachialis muscle. The most common sites for neurogenic HO are the hips, elbows (extensor side), shoulders, and knees. Uncommon sites of HO that may be encountered in a rehabilitation setting are incisions, kidneys, uterus, corpora cavernosum, and the gastrointestinal (GI) tract. The exact cause and mechanism of neurogenic HO are unknown. More will be discussed in the Pathophysiology section. Risk factors for developing HO are spasticity, older age, pressure ulcer, the presence of deep vein thrombosis (DVT), having a tracheostomy, long bone fractures, prior injury to the same area, edema, immobility, long-term coma, and severity of injury (trauma, TBI, SCI, stroke). High/moderate risk factors in the THA population include men with bilateral THA, prior history of HO, ankylosing spondylitis, diffuse idiopathic hyperostosis, or Paget's disease. 
In the total hip arthroplasty population, the incidence varies. In those with high-risk factors, incidence can be as high as 90%. Overall rates for developing HO in the THA population is closer to 53%. HO is twice as common in males versus females, but it is noted that females older than 65 years old have an increased risk of developing HO. The incidence of neurogenic HO is 10% to 20%. When differentiating between the incidence of HO in adult SCI patients is 20% to 30% and in adult TBI patients is 10% to 20% . HO in the pediatric TBI population ranges from 3% to 20%. 
The exact mechanism of HO in traumatic and neurogenic HO is unknown , but 2 common factors precede the formation of HO, the first being trauma or an inciting neurological event. In the SCI population, it is hypothesized that acute rehabilitation, transfer activities, and repeated microtrauma can add up during activities of daily living (ADL) can cause an accumulation of mechanical stress that predisposes one to the formation of HO. Second, after trauma or neurological injury, is the tissue expression of bone morphogenic proteins (BMPs). BMPs stimulate mesenchymal spindle stem cells, also known as satellite cells, to migrate to the injured area and transform into fibroblasts and eventually, osteoblasts. It has been proposed that alkaline phosphatase also plays a role in ectopic hone formation. Alkaline phosphatase acts to suppress inhibitors of bone formation is known to be elevated in vascular smooth muscle tissue in the presence of inflammatory cytokines and macrophages . After migration, mesenchymal spindle cells begin to differentiate into fibroblasts which then start to secrete immature connective tissue composed of collagen and extracellular matrix. With continued tissue irritation, fibroblastic metaplasia is activated, transforming fibroblasts into chondrocytes in a similar process to endochondral ossification. Some of the chondrocytes continue to deposit collagen into the cartilage matrix while the remaining chondrocytes transform into osteoblasts. By 1 to 2 weeks, new osteoid is present within the tissue, and new bone formation starts to form within the osteoid. Osteoblasts then begin to degrade and replace the cartilage with bone. Calcium pyrophosphate within the osteoid is slowly replaced with hydroxyapatite crystals as the bone mineralizes and and starts to mature. 
Microscopic examination of biopsy in myositis ossificans revealed an outer zone of hypercellular spindle cells and woven bone surrounding an inner zone of trapped muscle with normal osteoblasts continuing to lay down bone. To distinguish HO from osteosarcoma, HO is zonation from central immature fibrous tissue to peripheral mature bone is characteristic of HO. This helps distinguish HO from osteosarcoma, in which the central zone consists of mature bone . At initial injury, there will be a proliferation of hypercellular spindle cells. Cartilage and woven bone start to form 2 weeks after injury. Trabecular bone starts to form from weeks 2 to 5 with mature fatty bone marrow. After 6 weeks lamellar bone will mature. 
HO usually occurs 3 to 12 weeks after inciting injury , but can take up to 6 months to present. Look for a recent history of arthroplasty (total hip arthroplasty [THA], total knee arthroplasty), stroke, SCI, TBI, or burn. The most common presentation will be pain and decreased range of motion (ROM). Patients often complain of stiffness in their joints. Other common signs to look for are local edema, effusion, erythema, warmth, and tenderness  in the tissue or joint. Localized soft tissue swelling may present like a deep vein thrombosis (DVT). The patient may also present with a low-grade fever. Since spasticity is a risk factor, the patient may present with spasticity near the affected joint. Other risk factors to look for in the history are prolonged coma, tracheostomy or gastric tube, immobility, pressure ulcers and associated long bone fracture.  The greatest risk for developing HO also occurs at the 3 to 4 month period post-injury.
Alkaline phosphatase is historically the most commonly ordered lab, but may not be elevated early on in HO formation . It may take up to 2 weeks to be elevated and can rise to 3.5 times the normal value 10-weeks post-injury. Serum alkaline phosphatase greater than 250 has been shown to correlate with HO in the THA population, but there isn't a correlation between the level of alkaline phosphatase and the severity of the injury. Alkaline phosphatase can also be falsely elevated with associated long-bone injuries.
Erythrocyte sedimentation rate (ESR) is another inflammatory marker that is used. ESR greater than 35 mm/hr can indicate the development of HO . C-reactive protein is another inflammatory marker that can be elevated in early HO. Both are non-specific. Creatine kinase (CK) can be used to determine the severity of HO but is not a very specific test.  However, one study of 18 spinal cord injured patients determined that an elevated CK may be associated with a more aggressive course of HO and show possible resistance to etidronate therapy. 
Plain film radiographs show circumferential bone formation around or near a joint with a radiolucent center (figure 1). X-ray is specific for HO but not sensitive in early disease. Plain films may not be positive until 3 to 4 weeks after HO appears on bone scan. Therefore the triple phase bone scan is the most sensitive. A bone scan can reveal HO as early as 2.5 weeks after injury .
CT may be used to delineate the area of bone formation in preparation for surgical, but their role in evaluation/diagnosis of HO is not established . MRI may also be used but is not cost effective unless bone encompasses neurologic structures. Other imaging techniques uncommonly used are an ultrasound and 3-dimensional stereolithography.
The approach to prophylaxis involves identifying patients with high risk of developing HO. Routine prophylaxis is not recommended. Current recommendations for prevention of HO are gentle ROM exercises, indomethacin, etidronate, and external beam radiation, which is primarily used after joint arthroplasty. It is also important to manage risk factors, such as spasticity, in the prevention of HO. Indomethacin is the most commonly used NSAIDs used for prophylaxis . Others NSAIDs that have been proven effective are meloxicam, celecoxib, rofecoxib, and ibuprofen. The recommended dose of indomethacin for prophylaxis in the THA population is 75 to 100 mg per day for 7 to 14 days post-operatively. In the SCI population, 75 mg per day of indomethacin for 3 weeks is the recommended . Careful monitoring must be done for risk of bleeding, especially with concurrent chemoprophylaxis for venous thromboembolism. It is advised to use concurrent prophylaxis for gastrointestinal (GI) ulcers. Twenty mg per day of a selective COX-2 inhibitor can be used with fewer GI side effects compared to traditional NSAIDs .
Etidronate is a bisphosphonate that has been approved for prevention of heterotopic ossification in spinal cord injuries and complication of total hip arthroplasty. For spinal cord injury associated HO, the recommended treatment is 20 mg/kg per day for 2 weeks then 10 mg/kg per day for 10 weeks for a total treatment period of 12 weeks. For total hip arthroplasty, recommended treatment is 20 mg/kg per day for 1 month preoperatively and then the same dosing for 3 months postoperatively .
External beam radiation is most commonly used after total joint arthroplasty. A single dose of 700 to 800 g (centigray) is administered up to 24 hours preoperatively or within 72 hours postoperatively .
Current treatment recommendations consist of mobilization with ROM exercises, indomethacin, etidronate, and surgical resection.  Early treatment with a passive range of motion exercises should be implemented once the presence of HO is confirmed to prevent ankylosing of joints. Absolute treatment consists of surgical resection of mature bone once the HO has fully matured. This can be 12 to 18 months after the initial presentation . Surgical consultation with an orthopedic surgeon is warranted only if there will be an improvement in function as demonstrated by mobility, transfers, hygiene, and ADLs.
Indomethacin and etidronate are also used to help arrest bone formation in HO, but efficacy in the traumatic brain injury population has not been clearly proven. The most effective treatment option in the TBI population is surgical resection. In the SCI population, the most effective NSAID treatment regiments are either Rofecoxib 25 mg per day for 4 weeks or indomethacin 75 mg daily for 3 weeks. 
In the spinal cord injury population, DVT, cellulitis, abscess hematoma, and tumor (osteosarcoma, osteochondroma) are the most common differential diagnoses that should be ruled out, especially if plain film x-ray is negative. Other common differential diagnoses are hardware infection, thrombophlebitis, and osteomyelitis . A venous Doppler should be ordered to quickly and easily rule out a DVT. Cellulitis and abscess can be difficult to assess because white blood cell count (WBC) and inflammatory markers can be elevated in HO. Elevated alkaline phosphatase can help distinguish HO from other infectious conditions. CT scan/MRI with contrast can help differentiate HO from a hematoma, thrombophlebitis, osteomyelitis, and a tumor but is not the most cost-effective. Triple-phase bone scan remains the most sensitive for detection of HO, especially early on since it can take up to 6 weeks from the initial presentation of symptoms for HO to appear on an x-ray.
Staging is most often used for surgical planning and is not used much in rehabilitation management. Brooker Classification System, used for severity of HO in the hip, is the most commonly used staging system , which uses plain film radiograph to evaluate the presence and size of bone deposition visually. A simplified version has been proposed called the "Della Valle" classification system . None of the most commonly used rating systems take into account patient function or ROM, so a modified Brooker classification scale has been proposed that take these factors into account.
Brooker classification divides the extent of HO formation in the hip into 4 classes:
The "Della Valle" classification system :
Complications of HO present itself through decreased function and mobility, peripheral nerve entrapment, and pressure ulcers. Up to 70% of cases involving HA are asymptomatic. Ankylosis, vascular compression, and lymphedema can also be complications manifested in HO . Prognosis is generally good after surgery. Mean time from injury to surgery is 3.6 years. Once the surgery is performed, studies have shown that average ROM in the hip can improve from 24.3 to. After surgery, improvement was maintained in follow up 6 months after surgery. Complications from surgical resection of HO, such as infection, severe hematoma, and DVT .
General or orthopedic surgery should be consulted once the bone has matured and if HO is causing limitations in patients function, mobility, ability to provide self-care and perform ADLs or if the caretaker has a hard time performing ADLs, including hygiene and daily self-care.
HO is best managed by an interprofessional team that includes orthopedic nurses. The condition is not only difficult to diagnose because of lack of specific markers but its treatment is not satisfactory. Current treatment recommendations consist of mobilization with ROM exercises, indomethacin, etidronate, and surgical resection. Early treatment with a passive range of motion exercises should be implemented once the presence of HO is confirmed to prevent ankylosing of joints. Absolute treatment consists of surgical resection of mature bone once the HO has fully matured. Surgical consultation with an orthopedic surgeon is warranted only if there will be an improvement in function as demonstrated by mobility, transfers, hygiene, and ADLs.
|||Shehab D,Elgazzar AH,Collier BD, Heterotopic ossification. Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 2002 Mar [PubMed PMID: 11884494]|
|||Iorio R,Healy WL, Heterotopic ossification after hip and knee arthroplasty: risk factors, prevention, and treatment. The Journal of the American Academy of Orthopaedic Surgeons. 2002 Nov-Dec [PubMed PMID: 12470043]|
|||Hurvitz EA,Mandac BR,Davidoff G,Johnson JH,Nelson VS, Risk factors for heterotopic ossification in children and adolescents with severe traumatic brain injury. Archives of physical medicine and rehabilitation. 1992 May [PubMed PMID: 1580774]|
|||Chen HC,Yang JY,Chuang SS,Huang CY,Yang SY, Heterotopic ossification in burns: our experience and literature reviews. Burns : journal of the International Society for Burn Injuries. 2009 Sep [PubMed PMID: 19481867]|
|||Yoon BH,Park IK,Sung YB, Ankylosing Neurogenic Myositis Ossificans of the Hip: A Case Series and Review of Literature. Hip [PubMed PMID: 29896457]|
|||Rossier AB,Bussat P,Infante F,Zender R,Courvoisier B,Muhelm G,Donath A,Vasey H,Taillard W,Lagier R,Gabbiani G,Baud CA,Pouezat JA,Very JM,Hachen HJ, Current facts of para-osteo-arthropathy (POA). Paraplegia. 1973 May [PubMed PMID: 4576824]|
|||Al-Jarallah K,Al-Saeed O,Shehab D,Dashti K,Sheikh M, Ossification of ligamentum flavum in Middle East Arabs: a hospital-based study. Medical principles and practice : international journal of the Kuwait University, Health Science Centre. 2012 [PubMed PMID: 22687823]|
|||Mary Jiayi T,Linda P,Michael P,Hans K,Markku N,Richard J,Bo Angela W,May T,Elizabeth B,Edward C, Potential discrepancy between plain films and CT scans in Brooker classification of heterotopic ossification. The British journal of radiology. 2017 Dec [PubMed PMID: 28972793]|
|||Citak M,Suero EM,Backhaus M,Aach M,Godry H,Meindl R,Schildhauer TA, Risk factors for heterotopic ossification in patients with spinal cord injury: a case-control study of 264 patients. Spine. 2012 Nov 1 [PubMed PMID: 22614800]|
|||Kjaersgaard-Andersen P,Pedersen P,Kristensen SS,Schmidt SA,Pedersen NW, Serum alkaline phosphatase as an indicator of heterotopic bone formation following total hip arthroplasty. Clinical orthopaedics and related research. 1988 Sep [PubMed PMID: 3136963]|
|||Kjaersgaard-Andersen P,Schmidt SA,Pedersen NW,Kristensen SS,Pedersen P, Erythrocyte sedimentation rate and heterotopic bone formation after cemented total hip arthroplasty. Clinical orthopaedics and related research. 1989 Nov [PubMed PMID: 2509120]|
|||Sherman AL,Williams J,Patrick L,Banovac K, The value of serum creatine kinase in early diagnosis of heterotopic ossification. The journal of spinal cord medicine. 2003 Fall [PubMed PMID: 14997963]|
|||Joice M,Vasileiadis GI,Amanatullah DF, Non-steroidal anti-inflammatory drugs for heterotopic ossification prophylaxis after total hip arthroplasty. The bone [PubMed PMID: 29954195]|
|||Aubut JA,Mehta S,Cullen N,Teasell RW, A comparison of heterotopic ossification treatment within the traumatic brain and spinal cord injured population: An evidence based systematic review. NeuroRehabilitation. 2011 [PubMed PMID: 21447915]|
|||Ruo Redda MG,De Colle C,Bianco L,Ruggieri A,Nassisi D,Rossi A,Gino E,Airaldi C, Heterotopic ossifications: role of radiotherapy as prophylactic treatment. La Radiologia medica. 2018 Jun [PubMed PMID: 29397526]|
|||Behery OA,Dai AZ,McLaurin TM, Posttraumatic Heterotopic Ossification of the Hip. Journal of orthopaedic trauma. 2018 Aug [PubMed PMID: 29985897]|
|||Della Valle AG,Ruzo PS,Pavone V,Tolo E,Mintz DN,Salvati EA, Heterotopic ossification after total hip arthroplasty: a critical analysis of the Brooker classification and proposal of a simplified rating system. The Journal of arthroplasty. 2002 Oct [PubMed PMID: 12375245]|