Sexual Assault Evidence Collection

Earn CME/CE in your profession:

Continuing Education Activity

Sexual assault evidence collection is the process of collecting specimens and documenting injuries of sexual assault victims to be used in the court of law. Forensic examination for evidence collection is performed in emergency departments 90% of the time, and 10% in other locations such as urgent care, OBGYN, and primary care offices. Sexual Assault Nurse Examiners (SANEs) often perform evidence collection. This activity reviews the processes the healthcare team uses to collect evidence.


  • Describe the standard protocol for evidence collection.
  • Review the function of evidence collection.
  • Summarize the treatments that need to be performed concommitent with evidence collection.


Sexual assault evidence collection is the process of collecting specimens and documenting injuries of sexual assault victims to be used in the court of law. Forensic examination for evidence collection is performed in emergency departments 90% of the time, and 10% in other locations such as urgent care, OBGYN, and primary care offices.[1][2] Sexual Assault Nurse Examiners (SANEs) often perform evidence collection.[3]

 The protocol for evidence collection is as follows:

  1. Completion of consents and forms in the Sexual Assault Evidence Collection Kit (SAECK)
  2. A thorough history involving recent genital procedures, symptoms since the assault, details of the assault, number of assailants, specific threats, type of penetration, nongenital acts, loss of consciousness, amnesia, activities after the assault.
  3. Control swabs
  4. Toxicologic testing within 72 hours, especially if there is a loss of consciousness, to determine what drugs the patient used or ingested
  5. Blood or saliva swabs for patient’s DNA
  6. Oral swabs/smears if <24 hours since oral penetration
  7. Fingernail scrapings if the patient was able to scratch perpetrator
  8. Foreign material collection, which is material that falls off patients when undressing. Collect sheets on the exam table and/or from an ambulance as evidence may be present in the debris.
  9. Clothing collection. If cutting clothes off the patient, providers should pay special attention to preserve holes in clothing, stains that would corroborate the use of force against the patient.
  10. Examine the full body for injuries, lesions, secretions. Document with photos when possible. If bite marks are present, swab the area twice.
  11. Head-hair combings
  12. Pubic hair combings
  13. For female patients, examine for anogenital injuries in the lithotomy position. Take external genital swabs, vaginal swabs, perianal swabs.
  14. For male patients, examine penile and anal injuries. Take penile swabs, urethral swabs, anorectal swabs.
  15. Complete forms and seal envelopes inside the sexual-assault evidence collection kit with specimens.[3]

The examiner should go through each step with the patient before performing them and then allow the patient the opportunity to decline individual steps. The patient must provide written and verbal consent to the forensic exam, and this becomes problematic in the case of minors, elderly patients, and intoxicated patients.  The primary focus of the exam should be on treating injuries before collecting evidence. The process of evidence collection could take up to 6 hours by a trained professional. The optimal time frame for a forensic evaluation is within 72 hours of the assault to be able to collect as much DNA evidence as possible. However, a kit can still be useful in gathering evidence after this period, up to 7 days due to advancements in DNA technology. After this window of time, or if the patient does not give consent, only the physical and medical treatments are possible.[4]


SANE programs have extensive support across the United States and show demonstrably improved outcomes for their patients. Outcomes such as psychological recovery from the trauma, providing acute medical treatment, enhanced evidence collection, improved prosecution of sexual assault cases.[5] A study involving 515 evidence kits were audited and divided into two groups, SANE and non-SANE, according to who completed the kits. When comparing SANE to non-SANE, the SANE SAECKs were more likely to be correctly sealed (91% SANE vs. 75% non-SANE), to include the appropriate number of swabs (88% vs. 71%), to include the proper number of blood tubes (95% vs. 80%), and to maintain a completed chain of custody (92% vs. 81%).[6]

However, there are issues with the accessibility and experience of SANEs across the United States, especially in rural communities. Rural communities have high rates of sexual assault (some as high as 30%) where most research on forensic examinations takes place in urban settings.[7] These programs are also limited in rural areas. This situation causes patients to have to travel a long distance to a rural hospital that provides forensic exams or go to an urban area. Unfortunately, this creates a vicious cycle where patients need to travel to urban areas, but preliminary evidence suggests that telemedicine services for forensic examination may have a positive impact.[8] In rural areas, the lower caseload for SANEs places a burden on nurse examiners to find ways to maintain their competencies and skills. Also, it creates a burden on hospitals to staff and pay for an underutilized program.[7]

Another issue of concern with the forensic examination is the significance juries place on having DNA evidence. Since the arrival of crime scene investigation television shows, there has been this impression that collecting DNA evidence is easy and quick. This notion is wildly misleading; DNA evidence is usually challenging to obtain and takes an extensive amount of time to process. In a study on forensic evidence found in sexual assault cases, only about 55% of cases that reported penetration and ejaculation found spermatic material.[9] The processing of evidence also needs to be standardized for the best outcomes. Hospital laboratories may use alcohol or betadine to process samples, which may compromise the integrity of the report. Meanwhile, state laboratories where evidence is normally processed are aware of which chemicals to use and which to avoid during the processing.[10] While there are often other forms of forensic evidence available, attorneys are less likely to move forward with prosecution without significant DNA evidence.[7][11]

Pediatrics is another issue of concern since pediatric sexual assault is much less studied and cared for in the ED in comparison to adult sexual assault. Few SANE programs are designed with the pediatric patient in mind, and for this reason, some cases are not being cared for appropriately. Older children and young adults are more likely to receive appropriate STI and pregnancy screening and treatment than younger patients; this is likely due to the lack of protocol for treating pediatric patients in the forensic process. Also, due to a patient's age and developmental level, it may be difficult for a patient to consent and comply with a forensic exam.[12]

Issues of Concern

According to 2019 data collected by the US Department of Justice, sexual assault occurs every 73 seconds in the United States and every nine minutes for pediatric victims. The lifetime rates of sexual assault are between 17 to 18% for women and 3% for men. These numbers are likely under-estimations of the true values and do not account for rates of LGBT sexual assaults, for which the rates are higher. This demonstrates that sexual assault has a clinically significant impact on patients of which physicians, nurses, and other healthcare practitioners need to be cognizant, especially in the documentation and forensic examination of these patients. Particularly because a SANE response team is not available at every healthcare location, providers should be familiar with the process and locations that patients can receive a referral for examination.[13]

Initial evidence for SANE programs has suggested improved outcomes in patient psychological recovery, treatment, evidence collection, and prosecution of cases. However, there is still a need for more rigorous and standardized procedures for SANE program assessment.[5] A review comparing the historical control of no SANE practitioner to SANE practitioners in pediatric emergency departments found that there was improved quality of care found in cases managed by pediatric SANEs. This improvement included testing for STIs, documentation of injury, and assessment of pregnancy.[14]

Forensic examination demonstrates the intersectionality of medicine and law. One of the most multifaceted issues facing the medical and judicial systems is obtaining just outcomes for victims of sexual assault. Even with the availability of specialized forensic evidence collection, many survivors of sexual assault do not complete a sexual assault evidence kit, and even fewer release the evidence to police for investigation.[15] Emergency departments are a common entry point into the healthcare system, especially for cases of sexual assault, so this location is of extreme importance to the medical-judicial system. Evidence collected during the forensic exam has a significant role in legal decision making by the prosecution.[16]

Clinical Significance

The first step to treatment is taking care of the physical ailments. Once the patient is medically stable, measures are necessary for proper hospital monitoring and follow-up appointments.

During the emergency department visit or initial encounter, the patient is taken care of by way of pain control, antibiotic prophylaxis for STDs, nutritional services, and sleep improvement strategies. It is essential to monitor the patient’s emotional and psychological wellbeing by ordering a psychiatric consult. These victims are at higher risk of psychosocial disturbances such as PTSD, depression, sexual dysfunction, and chronic substance abuse.[4]

Follow-up psychiatric counseling is suggested, as the victim can experience psychological reactions as well as behavioral and somatic manifestations. This condition is known as rape trauma syndrome and is the immediate post-assault period that can present in up to 16% of patients.[17] In the acute phase, also called the disorganized phase, the victim may experience sleep disturbances, generalized physical pain, as well as mood and eating disturbances. The victim usually feels a sense of disorganization in their lives, with fear and blame being the predominant constituents. The delayed phase of sexual assault can include phobias, nightmares, flashbacks consistent with PTSD, but it can also result in sexual dysfunction, making intimacy challenging. Long-term consequences for these patients can be depression, chronic pelvic pain, and overall diminished quality of life; this is why it is imperative to have these patients follow up with a psychologist/psychiatrist who specializes in sexual assault cases. This process also includes screening for intimate partner violence, since over half of sexual assault cases happen from a partner.[4]

Other monitoring strategies involve following up with an OBGYN or PCP and ensuring any physical complaints are managed appropriately. During these follow-up appointments, the clinician can monitor any lingering infections or pregnancies, and give any appropriate vaccinations. An appointment two weeks after the initial assault should be scheduled to check for STIs, along with a 6-week appointment for repeat pregnancy testing, HIV screening, and hepatitis C. Subsequent appointments at 12 and 24 weeks should be made for follow-up HIV and hepatitis C testing.[4]

If the patient is pregnant at the time of the assault, an OBGYN should monitor the status of the fetus during the mother’s hospital stay. Gynecological trauma and physical abuse may have impacted the pregnancy.

Patients should receive an offer for legal counseling, even if they deny legal consultation at the time of the initial evaluation. Although most patients decide not to press charges, a well-documented account of the assault, as done during a SANE exam, can aid in prosecution.[18] The provider has a duty to ensure accurate and complete medical records, along with maintaining the chain of custody for evidence.[4]

In patients where drug-facilitation is suspected or confirmed, confirmation of the type of agent used is via a UDS. This process helps identify what type of hospital monitoring will be needed, as well as withdrawal tactics to use. The most common substances used in a drug-facilitated sexual assault are flunitrazepam, gamma-hydroxybutyrate (GHB), and ketamine, although other agents such as benzodiazepines and opioids are also possibilities.[4] It is important to monitor the patient and optimize their hospital medications as the drugs metabolize and leave the body.

Enhancing Healthcare Team Outcomes

Working as a healthcare team will obtain the best results in evidence collection and result in better outcomes for the patient. [Level 5]

Article Details

Article Author

Megan Ladd

Article Editor:

Jesus Seda


1/29/2023 7:52:43 AM



Campbell R,Townsend SM,Long SM,Kinnison KE,Pulley EM,Adames SB,Wasco SM, Organizational characteristics of Sexual Assault Nurse Examiner programs: results from the national survey project. Journal of forensic nursing. 2005 Summer;     [PubMed PMID: 17089484]


Strasburger D,Hall H,McCann N,Girzadas DV Jr, Emergency department medical evidence collection following sexual assault. Academic emergency medicine : official journal of the Society for Academic Emergency Medicine. 2010 Jan;     [PubMed PMID: 20078444]


Linden JA, Clinical practice. Care of the adult patient after sexual assault. The New England journal of medicine. 2011 Sep 1;     [PubMed PMID: 21879901]


Vrees RA, Evaluation and Management of Female Victims of Sexual Assault. Obstetrical     [PubMed PMID: 28134394]


Campbell R,Patterson D,Lichty LF, The effectiveness of sexual assault nurse examiner (SANE) programs: a review of psychological, medical, legal, and community outcomes. Trauma, violence     [PubMed PMID: 16217119]


Sievers V,Murphy S,Miller JJ, Sexual assault evidence collection more accurate when completed by sexual assault nurse examiners: Colorado's experience. Journal of emergency nursing: JEN : official publication of the Emergency Department Nurses Association. 2003 Dec;     [PubMed PMID: 14631337]


Annan SL, 'We desperately need some help here'--The experience of legal experts with sexual assault and evidence collection in rural communities. Rural and remote health. 2014;     [PubMed PMID: 25347043]


Walsh WA,Meunier-Sham J,Re C, Using Telehealth for Sexual Assault Forensic Examinations: A Process Evaluation of a National Pilot Project. Journal of forensic nursing. 2019 Jul/Sep;     [PubMed PMID: 31436683]


Tozzo P,Ponzano E,Spigarolo G,Nespeca P,Caenazzo L, Collecting sexual assault history and forensic evidence from adult women in the emergency department: a retrospective study. BMC health services research. 2018 May 29;     [PubMed PMID: 29843707]


Magalhães T,Dinis-Oliveira RJ,Silva B,Corte-Real F,Nuno Vieira D, Biological Evidence Management for DNA Analysis in Cases of Sexual Assault. TheScientificWorldJournal. 2015;     [PubMed PMID: 26587562]


Wentz EA, Funneled Through or Filtered Out: An Examination of Police and Prosecutorial Decision-Making in Adult Sexual Assault Cases. Violence against women. 2019 Dec 23;     [PubMed PMID: 31868129]


Goyal MK,Mollen CJ,Hayes KL,Molnar J,Christian CW,Scribano PV,Lavelle J, Enhancing the emergency department approach to pediatric sexual assault care: implementation of a pediatric sexual assault response team program. Pediatric emergency care. 2013 Sep;     [PubMed PMID: 23974714]


Mollen CJ,Goyal MK,Frioux SM, Acute sexual assault: a review. Pediatric emergency care. 2012 Jun;     [PubMed PMID: 22668668]


Hornor G,Thackeray J,Scribano P,Curran S,Benzinger E, Pediatric sexual assault nurse examiner care: trace forensic evidence, ano-genital injury, and judicial outcomes. Journal of forensic nursing. 2012 Sep;     [PubMed PMID: 22925125]


Muldoon KA,Drumm A,Leach T,Heimerl M,Sampsel K, Achieving just outcomes: forensic evidence collection in emergency department sexual assault cases. Emergency medicine journal : EMJ. 2018 Dec;     [PubMed PMID: 30089612]


Kjærulff MLBG,Bonde U,Astrup BS, The significance of the forensic clinical examination on the judicial assessment of rape complaints - developments and trends. Forensic science international. 2019 Apr;     [PubMed PMID: 30797159]


Burgess AW,Holmstrom LL, Rape trauma syndrome. The American journal of psychiatry. 1974 Sep;     [PubMed PMID: 4415470]


Tintinalli JE,Hoelzer M, Clinical findings and legal resolution in sexual assault. Annals of emergency medicine. 1985 May;     [PubMed PMID: 3985466]